Anti-Human RAGE Recombinant Antibody (XT-M4)
CAT#: TAB-293CT
Recombinant monoclonal antibody XT-M4 specifically binds to RAGE and neutralizes RAGE-associated activities. This antibody can be potentially used for treating RAGE-related disease.
Published Data
Tested Data
Gene Expression
![Figure 1 Radioactive equivalent concentrations and total radioactivity, as percentage of administered dose (% dose) of [125I]anti-RAGE Ab in serum, lung and spleen of RAGE-/- and wild-type (129S5) mice following a single 5 mg/kg IV dose.](https://img.creativebiolabs.net/productimages/TAB-293CT-1.png)
PK
Figure 1 Radioactive equivalent concentrations and total radioactivity, as percentage of administered dose (% dose) of [125I]anti-RAGE Ab in serum, lung and spleen of RAGE-/- and wild-type (129S5) mice following a single 5 mg/kg IV dose.
Serum and tissue RE concentrations were monitored by gamma-counting up to ∼5 mo (132 days) in RAGE-/- (open circles) and wild-type (wt) 129S5 (open triangle) mice. The insert in shows serum RE concentrations up to day 9 post-dose. Data show mean ± SD values, with n = 6 per time point. Individual animal concentration values that were less than the limit of quantitation were treated as zero for calculations of the mean and the standard deviation.
Vugmeyster, Y., DeFranco, D., Pittman, D. D., & Xu, X. (2010, September). Pharmacokinetics and lung distribution of a humanized anti-RAGE antibody in wild-type and RAGE-/-mice. In MAbs (Vol. 2, No. 5, pp. 571-575). Taylor & Francis.
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![Figure 1 Radioactive equivalent concentrations and total radioactivity, as percentage of administered dose (% dose) of [125I]anti-RAGE Ab in serum, lung and spleen of RAGE-/- and wild-type (129S5) mice following a single 5 mg/kg IV dose.](https://img.creativebiolabs.net/productimages/TAB-293CT-1.webp)
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WB
Figure 1 Anti-Human RAGE Recombinant Antibody (TAB-293CT) in WB
WB analysis of TAB-293CT was performed by coating with human RAGE protein.
TAB-293CT incubation concentration: 2 ng/μL.
Lane1: Reducing antigen (0.1μg)
Lane1: Reducing antigen (0.3μg)
Lane1: Reducing antigen (0.6μg)
The secondary antibody: HRP-Anti-Human IgG (H+L)
ELISA
Figure 2 Anti-Human RAGE Recombinant Antibody (TAB-293CT) in ELISA
ELISA analysis of TAB-293CT was performed by coating with recombinant human RAGE protein.
The secondary antibody: HRP-Anti-Human IgG (H+L)
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Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli fibrillar center & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64436/1232_G12_3_selected.jpg
Normal Tissue
Figure 2 Lung
Alveolar cells type I
Staining: High
Intensity: Strong
Quantity:>75%
Alveolar cells type II
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69474/168057_A_2_4.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (HEK93, JURKAT, RPTEC TERT1, U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204305-AGER
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Specifications
- Immunogen
- Human RAGE-Fc
- Host Species
- Humanized
- Type
- Humanized antibody
- Specificity
- Human
- Species Reactivity
- Human, mouse
- Clone
- XT-M4
- Applications
- WB, ELISA, Neut, PK
- Related Disease
- RAGE-related disease
Applications
- Application Notes
- The RAGE antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Neutralization, Pharmacokinetic.
Target
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Versatile Research Tool
XT-M4 has become a cornerstone in our lab's study of inflammatory pathways. Its specificity and high sensitivity to RAGE offer us consistent results across ELISA and Western Blot assays. We are particularly impressed by its batch-to-batch consistency, which greatly supports our longitudinal studies.
Great for Chronic Disease Research
As part of a diabetes inflammation project, XT-M4 has provided accurate insights. Its interaction with RAGE-a critical player in insulin resistance-sheds light on inflammation management. The antibody's robust production processes mean we can depend on its consistency and clarity in results.
Exeptional ELISA Performance
This antibody demonstrates superior performance in ELISA assays, with strong binding to recombinant human RAGE protein. The assays are more reliable, giving us the confidence needed for high-stakes projects. Its animal-free production is a bonus for sustainability-focused labs.
Q&As
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What is the primary application of this antibody?
A: The Anti-Human RAGE Recombinant Antibody (XT-M4) is primarily used in research for applications such as Western Blot, ELISA, Neutralization, and Pharmacokinetics. It is designed to target the RAGE (Receptor for Advanced Glycation End-products) protein involved in many pathological states related to inflammation and chronic diseases.
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Which diseases are associated with RAGE?
A: RAGE is a significant biomarker in multiple diseases, including diabetes, Alzheimer's disease, and various inflammatory conditions. The presence of RAGE is often linked with chronic inflammatory responses and oxidative stress.
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How does the antibody neutralize RAGE-associated activities?
A: By specifically binding to RAGE, XT-M4 neutralizes its activity, thereby inhibiting downstream signaling pathways involved in inflammatory responses and chronic disease progression.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-293CT, RRID: AB_3111903)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
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